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KMID : 0359320000400030601
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Korean Journal of Veterinary Research
2000 Volume.40 No. 3 p.601 ~ p.609
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Development of an ELISA kit for the detection of residual sulfadimethoxine in edible animal products
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김우택/Kim, Woo Taek
김성희/임윤규/윤병수/Kim, Seong Hee/Lim, Yoon Kyu/Yoon, Byoung Su
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Abstract
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An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%.
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